使用了先前被定向插入GFP报告基因的ES细胞。靶向载体去除了尾部调控元件的胸腺增强子(TE),但保留了沉默序列。在沉默元件附近插入了含荧光素基质诺卡因的选择标记。最终,通过暂时的Cre表达,删除了耐药基因座。(来源:J:193321)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129 x C57BL/6)F1
Targeted
基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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