Zbtb7b^tm5.1Itan

ES cells were used that had been previously targeted for the insertion of a GFP reporter. The targeting vector deleted the thymic enhancer (TE) in the distal regulatory element while leaving the silencer sequence intact. A floxed neomycin selection was also inserted adjacent to the silencer element. The resistance cassette was eventually excised by transient cre expression. (J:193321)