Tg(CMV-LITAF*W116G)#Lli

The N-terminal hemagglutinin (HA)-tagged human LITAF with a W116G mutation is under the control of the human cytomegalovirus promoter. Three lines were made and this one is the highest expressing line. Immunoblot analysis of sciatic nerve and Schwann cell lysates confirmed expression and genome walking PCR and sequencing identified that the transgene inserted in a non-coding region on chromosome 14 between Thrb and Nr1d2. The pound symbol (#) is used when line is not specified. (J:194981)