Tg(UBC-PA-GFP)1Mnz

This transgene comprises 1.2 kb of 5'-flanking DNA (nucleotides -1225 to -6) of the human ubiquitin C gene, which is expressed in a broad range of cell types, including all hematopoietic cells, driving expression of a photoactivatable green fluorescent protein cDNA, which is followed by both the SV40 and the bovine growth hormone polyadenylation signals. PA-GFP differs from EGFP by the substitution of histidine for threonine at amino acid position 203 (T203H); this modification results in ~100-fold enhanced fluorescence of PA-GFP upon excitation at 488 nm after preliminary photoactivation with intense 413-nm light, greatly increasing the optical contrast. (J:33045, J:93490, J:166836, J:195187)