Tg(Mt1-SERPINE1)1Upj

This transgene comprises an 1,800 bp DNA fragment containing the mouse metallothionein 1 promoter followed by a 1,397 bp cDNA encoding human SERPINE1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; also called endothelial cell plasminogen activator inhibitor 1) and the bovine growth homone polyadenylation signal. Whereas SERPINE1 mRNA is elevated in livers of 4 day old transgenic mice, by 28 days it has dropped to control levels. Four day-old transgenic mice have greatly elevated levels of the protein, as measured by enzyme-linked immunosorbent assay (ELISA) using a monoclonal anti-SERPINE1 antibody, in spleen, liver and kidney, and lower, but still markedy elevated levels in heart, brain and lung. The liver protein level has diminished >50% by 7 days and 10-fold by 28 days, but remains ~3.5-fold that of controls. Injection of 28 day old mice with zinc sulfate (ZnSO4) induces expression of the protein in plasma (20-fold increase) and in all tissues analyzed. (J:194516)