Gclc^{tm1c(EUCOMM)Wtsi}

The L1L2_gt0 cassette was inserted at position 77681930 of Chromosome 9 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 77682710. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ/neo cassette and left exon 2 floxed. (J:194778)