在exon 2的上游插入了一个loxP位点。随后,一个由FRT引导的基因捕获盒(包含拼接接受器、neo标记、IRES、GFP和polyadenylation序列)带有3'的loxP位点,被定向插入到exon 3的下游。通过Flp介导的重组,基因捕获载体被去除,剩下的exons 2和3被标记为可剪接。来源:(J:194195)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129
Targeted
插入
--
1
4
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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