靶向载体包含NEO cassette和polyA序列,它们能打乱12到15号外显子以及16号部分外显子, Western blot结果显示大脑中没有蛋白质表达(来源:J:194306)
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A targeting vector contains a NEO cassette and polyA sequence that disrupt exons 12 through 15 and a portion of exon 16. Western blot analysis confirmed the absence of protein expression in the brain. (J:194306)