这个靶向载体包含一个包含一个剪接接受器和正常15-17号外显子序列的"迷你cDNA",后面跟着一个FRT标记的neo cassette和第三个loxP位点,插入在14号内含子中,以及在插入点3'端的D620N突变。迷你cDNA的polyadenylation信号序列阻止了D620N突变的表达。通过Flp介导的重组,neo cassette被移除了。(来源:J:274797)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6.Cg-Thy1a
Targeted
插入
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1
2
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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