Tg(LRRK2*R1441G)3IMjff

A human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was modified by targeted mutation of the LRRK2 locus to harbor the LRRK2 R1441G (4321 C>G) mutation associated with autosomal dominant, late-onset Parkinson's disease originally identified in Spanish families originating from the Basque region. Transgene insertion occurred on Chr 1, causing a 39,855 bp deletion. (J:101977)