在9a内插入了loxP位点和FRT引导的诺姆西林筛选 cassette,随后在3'非编码区的exon 13中插入了另一个loxP位点。之后,通过flp重组酶,将这个正确靶向的等位基因中的筛选 cassette移除。从exon 10到exon 13的编码区域,两侧都被loxP位点所环绕。Cre的剪切会去除包括膜内域和含有FERM和PDZ蛋白结合区域在内的C端细胞内域。(来源:J:191149)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
3
13

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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