大部分exon 3被一个floxed neo cassette替换,随后通过Cre介导的重组移除了neo cassette。 Western blot结果显示肾组织中没有蛋白表达(来源:J:191142)
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Most of exon 3 was replaced with a floxed neo cassette. Cre-mediated recombination removed the neo cassette. Western blot analysis confirmed the absence of protein expression in the kidney. (J:191142)