在编码外显子的第30个核苷酸位置,插入了COIN模块(包含L71 loxP位点、倒置poly(A)序列、倒置的EGFP、L66 loxP位点和FRT引导的neo cassette)。这个插入导致了CpG岛的分割。通过Flp介导的重组,neo cassette被去除了。要表达EGFP,需要Cre介导的重组。逆转录PCR确认Sox2ot和Mir1897的正常转录表达。(来源:J:191980)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6NTac)F1
Targeted
插入
--
1
6
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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