用于产生初始Hdc-cre等位基因的靶向载体经过改造,插入了在Hdc第12外显子的终止密码子后,一个包含IRES2i-EGFP-IRES2i-cre-FRT-neo-FRT的序列。这个结果产生的等位基因是三重剪接型,能编码Hdc、EGFP和cre。与Flp敲除小鼠杂交去除了neo标记。然而,从这种mRNA构型中,cre的翻译并不足以驱动靶基因序列的重组。(来源:J:188103)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入
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1
1
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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