通过Cre介导的重组,Vdrtm1.1Sgcg这个不含neo cassette的靶向序列去除了exon 4。 Western blot结果显示尾巴区域没有检测到蛋白表达(来源:J:189471)
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Cre-mediated recombination of Vdrtm1.1Sgcg, which lacks the neo cassette, removed exon 4. Western blot analysis confirmed the absence of protein expression in the tail. (J:189471)