在第2外显子上游插入了一个带有5' loxP位点的FRT引导的neo cassette,接着在第3外显子下游插入了另一个loxP位点。 Western blot分析证实了小肠中没有蛋白表达(来源:J:190580)
查看原文 参与反馈
An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 3. Western blot analysis confirmed the absence of protein expression in the intestine. (J:190580)