整个开放阅读框,由单一外显子编码,被替换为一个EGFPf-2A-FLP-ACN构建。Farnesyl化的EGFP(EGFPf)通过牛痘病毒2A肽(FMDV 2A或F2A)序列与FLPo(优化的FLP重组酶)连接,然后与自我剪接的loxP引导的polymerase II启动子-neomycin抗性 cassette(ACN)连接。通过Cre介导的重组,删除了neo cassette。(来源:J:194547)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入,基因内删除
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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