整个开放阅读框,由单一外显子编码,被替换为一个EGFPf-2A-FLP-ACN构建。Farnesyl化的EGFP(EGFPf)通过牛痘病毒2A肽(FMDV 2A或F2A)序列与FLPo(优化的FLP重组酶)连接,然后与自我剪接的loxP引导的polymerase II启动子-neomycin抗性 cassette(ACN)连接。通过Cre介导的重组,删除了neo cassette。(来源:J:194547)
Basic Information
129S1/Sv-Oca2+ Tyr+ Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count