通过Cre介导的重组,去除了neo cassette、exon 7和exon 8。 Western blot结果显示,用表达Cre的腺病毒处理后,小鼠胚胎成纤维细胞中没有蛋白表达(来源:J:189563)
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Cre-mediated recombination removed the neo cassette, exon 7 and exon 8. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts following treatment with a cre-expressing adenovirus. (J:189563)