人类的cDNA被插入到了exon 4中。通过Cre介导的重组,下游的floxed neo cassette被去除了。 Western blot结果显示B细胞中未检测到小鼠蛋白表达。(来源:J:189017)
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The human cDNA was inserted into exon 4. Cre-mediated recombination removed the downstream floxed neo cassette. Western blot analysis confirmed the absence of murine protein expression in B cells. (J:189017)