Cre介导的重组移除了exon 1,保留了neo cassette。 Western blot分析证实了棕色脂肪组织、阴囊白色脂肪组织和肝脏中没有蛋白质表达(来源:J:189018)
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Cre-mediated recombination removed exon 1 and left the neo cassette intact. Western blot anlaysis confirmed the absence of protein in the brown adipose tissue, epididymal white adipose tissue and liver. (J:189018)