Grhl3^{tm2.1(F2rl1)Cgh}

A cDNA encoding the mouse F2rl1 was inserted in frame into the ATG start codon. An internal ribosomal entry sequence (IRES) followed by a nuclear localization signal and lacZ gene were introduced downstream of the F2rl1 gene. An FRT-flanked Neo cassette was positioned 5' of the F2rl1. Chimeric males derived from blastocyst injections of positive clones into C57BL/6 recipients were mated with C57BL/6 FLPe females to remove the neo gene. (J:189278)