Tg(tetO-IL1B)KBry

This transgene contains the human IL1B cDNA coding for the active, mature protein which was inserted between seven copies of the tetracycline Operator (tetO)7-CMV minimal promoter and the bovine growth factor polyadenylation signals sequence. TetO, when present in cells also carrying a tetracycline transactivator (tTA) or reverse transactivator (rtTA) transgene, is inactivated or activated, respectively, by the administration of tetracycline or its analog doxycycline. Three lines were made (K,L, and M) with line K showing highest human IL1B levels. (J:107601)