在exon 4上游插入了loxP位点和FRT引导的neo cassette,通过同源重组,又在exon 6下游插入了loxP位点。通过重组酶介导的重组,neo cassette和4-6号外显子被移除。免疫印迹分析证实了纯合小鼠MEFs中没有蛋白表达。(来源:J:188802)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count