通过同源重组,部分exon 1和全部exon 2被lacZ和pgk-neo的插入片段替换。 Western blot结果显示,纯合小鼠皮肤和睾丸的提取物中没有蛋白质表达。(来源:J:188696)
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Part of exon 1 and all of exon 2 were replaced with lacZ and pgk-neo cassettes via homologous recombination. Western blot analysis confirmed the absence of protein expression in lysates from skin and testes of homozygous mice. (J:188696)