通过Cre介导的重组,Gjb2tm3.1(Gjb1)Kwi中去除了floxed lacZ,从而允许了插入序列的表达。 Western blot结果显示,胚胎皮肤和胎盘中蛋白质表达显著减少。(来源:J:188626)
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Cre-mediated recombination of Gjb2tm3.1(Gjb1)Kwi removed the floxed lacZ and allowed for expression of the inserted coding sequence. Western blot analysis confirmed reduced protein expresion in embryonic skin and placenta. (J:188626)