从2号到5号外显子被替换成了一个由FRT和loxP序列引导的neo cassette。 Western blot结果显示大脑中没有检测到蛋白质表达。(来源:J:187673)
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Exons 2 through 5 were replaced with an FRT- and loxP flanked neo cassette. Western blot analysis confirmed the absence of protein expression in the brain. (J:187673)