FAST(Flexible Accelerated STOP Tetracycline Operator)技术被用来构建一个包含loxP位点、FRT位点、neo cassette、STOP、FRT位点、tetracycline operator(tetO)以及位于起始翻译位点上游的loxP位点的构造。通过重组,这个neo/STOP cassette会被去除,从而实现内源基因在tetO驱动下的表达。(来源:J:159353)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
插入
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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