使用了Pdx1tm1Mgn的重组酶介导 cassette 交换(RMCE)技术,插入了一个FRT标记的hygro cassette在区域IV的上游,同时将核定位的CFP插入到起始密码子位置,替换部分exon。通过Flp介导的重组,移除了hygro cassette。区域IV的上游保留了一个lox66/71位点,而CFP的下游则有一个lox2272位点。(来源:J:187872)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
插入,基因内删除
--
1
8
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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