一段大约3.1kb的基因组片段,包括6号和7号外显子,通过同源重组被一个带有floxed neo cassette的替换单位替换。 Western blot结果显示,纯合小鼠大脑中没有检测到蛋白表达。(来源:J:186718)
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An approximately 3.1 kb genomic fragment including exons 6 and 7 was replaced with a floxed neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in the brains of homozygous mice. (J:186718)