通过同源重组插入新 cassette,导致 exon 6 的中断。RT-PCR 和 Western blot 的结果显示,纯合小鼠组织中没有 mRNA 和蛋白质表达(来源:J:187109)
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Insertion of a neo cassette via homologous recombination disrupted exon 6. RT-PCR and western blot analysis confirmed the absence of mRNA and protein expression in tissues from homozygous mice. (J:187109)