Cre重组酶被插入到Etl4(Skt)基因座的第14个内含子中,通过使用带有左元件/右元件(LE/RE)突变Lox站点的可交换基因陷阱系统,实现了通过重组酶介导的 cassette exchange。原本陷阱载体中的IRES-cre序列被替换成了IRES-lacZ-neo功能单元,通过将载体和pCAGGS-cre质粒一起电穿孔到含被陷阱的Etl4基因的TT2 ES细胞中实现这一操作。(来源:J:187217)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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