设计了一个靶向载体,用于将内源性角蛋白17(Krt17)基因的终止密码子替换为(从5'至3')一段病毒2A多肽(以促进核糖体跳过),Cre重组酶,另一个2A多肽, cerulean绿色荧光蛋白(Cerulean-GFP)的变异版本,以及牛的poly(A)序列。随后是Frt标记的诺卡因抗性基因座(neo)。这个载体被电穿孔注入了C57BL/6NTac衍生的JM8A3胚胎干细胞。(来源:J:187143)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N-Atm1Brd
Targeted
插入,基因内删除
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1
5
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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