Lrp1^tm6Ajmr

Lrp1 knock-in mouse carrying mutant Lrp1 cDNA sequences in the 3' end of the gene replacing the corresponding genomic sequences: no intron 76 to intron 88 sequences present. The mutations are introduced to generate a stop codon in front of the distal NPXYXXL (NPXY2) motif of the intracellular domain of LRP1 (PTNFTNPVYATL - PTNFT*). The Lrp1 knock-in allele was generated by application of recombinase mediated cassette exchange (RMCE) in a ES cell line carrying an exchangeable cassette with a selection marker in the Lrp1 gene replacing the 3' end exons (exons 76 to 89) of the gene. By application of RMCE the Lrp1 gene was functionally restored by wild-type or mutant Lrp1 cDNA sequences resulting in a series of Lrp1 knock-in mutant alleles and mice including the Lrp1 knock-in mutant with a stop codon introduced in front of the NPXYXXL (NPXY2) motif. (J:186908)