Lrp1^tm5Ajmr

Lrp1 knock-in mouse carrying mutant Lrp1 cDNA sequences in the 3' end of the gene replacing the corresponding genomic sequences: no intron 76 to intron 88 sequences present. The mutations are introduced to inactivate the proximal NPXY (NPXY1) motif (NPTY - AATA) and the distal NPXYXXL (NPXY2) motif (NPVYATL - AAVAATL) in the intracellular domain of LRP1. The Lrp1 knock-in allele was generated by application of recombinase mediated cassette exchange (RMCE) in a ES cell line carrying an exchangeable cassette with a selection marker in the Lrp1 gene replacing the 3' end exons (exons 76 to 89) of the gene. By application of RMCE the Lrp1 gene was functionally restored by wild-type or mutant Lrp1 cDNA sequences resulting in a series of Lrp1 knock-in mutant alleles and mice including the Lrp1 proximal NPXY (NPXY1) motif and distal NPXYXXL (NPXY2) motif double knock-in mutant. (J:186908)