设计了一种靶向载体,将增强型绿色荧光蛋白(EGFP)插入到第3'端的外显子3后面。下游位置插入了一条由FRT和loxP序列标记的诺卡因抗性基因 cassette (neo)。通过Cre介导的重组,去除了neo cassette。(来源:J:186832)
Basic Information
(C57BL/6J x 129S4/SvJae)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count