Tg(Crx-cre)764Gla

A targeting construct containing a cre-FRT-amp-FRT cassette with an SV40 polyA sequence was used to target the Crx gene in BAC clone RP23-81H17 by recombineering methods in bacteria. The cassette was inserted after the ATG initiation codon in exon 2. The amp selection cassette was deleted in targeted clones. The purified BAC-cre construct was injected into fertilized oocytes. More than 3 lines were generated and line 60 was more extensively characterized. (J:186563)