Tg(Crx-Atoh7,-cre)60Gla

A BAC transgenic targeting construct containing an Atoh7-IRES-cre-FRT-amp-FRT cassette with an SV40 polyA sequence was used to target the Crx gene in BAC clone RP23-81H17 by recombineering methods in bacteria. The cassette was inserted after the ATG initiation codon in exon 2. The amp selection cassette was deleted in targeted clones. The purified BAC construct was injected into fertilized oocytes. More than 3 lines were generated and line 764 was more extensively characterized. (J:186563)