Tg(CAG-Cfh*)#Mcp

A modified version of the CAGGS promoter drives expression of a mouse Cfh gene with a Cys937Stop mutation resulting in a truncated protein that lacks the terminal five SCR domains. The truncated protein is detected in the plasma at levels comparable to the wild-type protein. The truncated protein retained complement regulatory activity but showed impaired binding to heparin and human umbilical vein endothelial cells in vitro. (J:125860)