App^tm1Yah

A MICER vector was used to insert a puromycin resistance cassette, a loxP site and the 3' half of an Hprt minigene between exons 16 and 17 of the amyloid precursor protein (App) gene in ES cells already bearing Runx1tm1Yg, which contains a loxP-flanked neomycin resistance cassette upstream of exon 2 of the runt related transcription factor 1 (Runx1) gene. Apptm1Yah resulted from the vector's integration into the same copy of Chr 16, i.e., in cis with Runx1tm1Yg. All loxP sites are directed distally on the chromosome. (J:185269)