Rab38^em3Rkuhn

Zinc finger endonuclease mediated homologous recombination introduced nucleotide substitutions to restore the wild-type coding sequence at codon 19 of the spontaneous Rab38 mutant allele. Additionally, non-homologous end joining resulted in a 27 bp deletion that disrupts the ApaLiI restriction endonuclease site at codons 30 and 31. (J:185512)