Tg(Msx3-icre)1/1Wdr

A targeting vector was used to insert a codon improved cre recombinase (icre) and an Frt-flanked chloramphenicol selection cassette into exon 1 of the Msx3 locus in a phage artificial chromosome (PAC 402-G09) by homologous recombination. This resulted in fusing the icre sequence to the ATG of Msx3. The selection cassette was removed by induction of Flpe in EL250 cells, and the purified vector was used for pronuclear injection. 8 founders were obtained. Of these 2 were studied in more detail (1/1 and 3/2), displaying identical patterns of cre expression and GFP expression when crossed to ROSA-GFP reporter mice. Line 1/1 was used for derivation of animals for experimental study. (J:186011)