Recombinase-mediated cassette exchange (RMCE) replaced the floxed hygro cassette in Gt(ROSA)26Sortm1Npa with an EEF1A1 promoter, EGFP and FRT-flanked neo cassette. EGFP is detected in all organs analyzed (salivary glands, heart, pancreas, liver, kidney, fat, muscle, brain and testis) and strong levels in testis. (J:184312)