Tg(NPHS2-Trpc6*E896K)F75aWalz

The Trpc6-HA wildtype cDNA sequence was made by modifying a Trpc6 cDNA sequence (encoding a 930 amino acid protein) originally isolated from mouse brain tissue by both repairing an A2615G point mutation (D872G) and also by in-frame addition of an influenza virus hemaglutinin epitope (HA-tag) at the 3' end. The cDNA sequence was placed downstream of a 2.5 kbp fragment from the promoter/enhancer region of the human podocin locus (NPHS2) and upstream from a bovine growth hormone polyA sequence. The G2686A nucleotide substitution was introduced to produce the amino acid substitution of lysine for glutamic acid at position 896 (E896K). Lines F73a and F75a were generated. (J:165112)