在E12.5期胚胎中,通过同源重组将包含IRES lacZ报告基因和诺卡因抗性基因的盒式载体插入了exon 1, Western blot结果显示没有检测到蛋白表达。(来源:J:183486, J:202343)
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A cassette containing a IRES lacZ reporter construct and neomycin resistance gene was inserted in exon 1 via homologous recombination. Western blot analysis confirmed the absence of protein expression in E12.5 embryos. (J:183486, J:202343)