Tg(Fabp1-NTN1)3Mhln

This transgene employs the Fabpl4x at -132 promoter cassette from the pTS4 expression vector to drive expression of a full-length cDNA encoding chicken netrin 1 (NTN1) with a C-terminal Myc epitope tag (EQKLISSEEDL). The promoter cassette contains nucleotides (nt) -596 through +21 of the rat fatty acid binding protein 1, liver (Fabp1) gene with an insertion at nt -132 -- just upstream of its endogenous position -- of four additional tandem copies of the 35-bp putative nuclear protein binding site, composed of a direct repeat of the sequence TTCTGNNTT separated by five nts, that resides at nts -172 to -133 of the Fabpl gene. (J:39591, J:92659)