Tg(Prism)1849Htz

Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. (J:189629)