Cre介导的重组移除了2到4号外显子以及neo cassette。 Western blot结果显示四头肌、胸三头肌、股二头肌、膈肌、大脑、肾脏和肺部均无蛋白表达。(来源:J:182571)
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Cre-mediated recombination removed exons 2 through 4 and the neo cassette. Western blot analysis confirmed the absence of protein expression in the quadriceps, triceps, gastrocnemius, diaphragm, brain, kidney and lung. (J:182571)