Bbs7^tm1Vcs

Exon 5 was replaced with a neomycin cassette. This causes a frameshift resulting in a null allele. The absence of expressed transcript was verified by RT-PCR analysis using RNA isolated from homozygous mutant mouse testes with primers within exon 5. The absence of the encoded protein was confirmed by western blotting of homogenates of homozygous mutant mouse testes. (J:226740)