将含有Hoxd11-IRES2-nlslacZ的磁带通过同源重组插入到YFP3.1胚胎干细胞的Gt(ROSA)26Sor位点。插入的磁带前面有一个由cre介导的剪切位点,除非经过剪切,否则会阻止Hoxd11或lacZ的转录。靶向的胚胎干细胞注射到C57BL/6J囊胚中。该遗传背景的细胞系保留在C57BL/6J背景上。(来源:J:137524)
Basic Information
129 and C57BL/6 and CBA and Swiss Webster
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count