通过Cre介导的重组,neo cassette和Mir205tm1Mtm的微小RNA茎环结构被移除。qRT-PCR结果显示微小RNA茎环确实丢失了。在E11.5时,胚胎前脑、咽弓和四肢中观察到了β-galactosidase表达,而在E18.5时,胚胎的心脏、皮肤和肾脏也显示出这种表达(引用号:J:181752)。
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count