一段经过修改的全长小鼠Sox6基因,其C端添加了人类MYC标签,被克隆到了pEFneo载体的下游,这个载体带有人类真核延伸因子1α1启动子。通过Northern blot和Western blot实验,用Sox6基因探针检测和用MYC抗体检测,我们发现1号品系的小鼠在肝、肌肉和T淋巴细胞中,外源基因产生的mRNA和蛋白质表达量分别是野生型小鼠内源基因的5倍。(来源:J:181312)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129P2/OlaHsd
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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